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Cooled the reaction mixture to 25-30C

In the above purification process, the buffer solution and the alcohol solvent are selected from those described in ninth embodiment of the present invention

The C18 column was washed with Acetonitrile: Water and then equilibrated with aqueous Ammonium acetate + aqueous ammonia

The seventeenth embodiment of the present invention provides a method of treating or preventing a disease comprising administering to the patient a therapeutically effective amount of Semaglutide obtained as per the process of the present invention

Women who maintained consistent supplementation (80% adherence) experienced 24% fracture risk reduction -- twice the benefit of inconsistent users